The HI84500 determines the free and total sulfur dioxide concentration in wine using the Ripper method. Excess iodide is added to the wine sample and reacts with the iodate titrant to produce iodine:
IO3- + 5I- + 6H+ → 3I2 + 3H2O
The iodine produced reacts with sulfur dioxide in the wine according to the redox reaction below:
H2SO3 + I2 → H2SO4 + 2HI
The HI84500 mini titrator is designed to determine free and total sulfur dioxide levels in wine by potentiometric titration in both a low range (1.0 to 40.0 ppm of SO2) and high range (30 to 400 ppm of SO2). The HI84500 can be used with all wines, including red, which are difficult to test using traditional methods due to the difficulty to observe a distinctive color change when the endpoint is reached.
Analysis of sulfur dioxide in wine, as performed on the HI84500 mini titrator, utilizes a simple sample preparation, a high quality titrant dosing pump, potentiometric endpoint detector, and automatic computations. To maintain the high precision of the titrator, a simple pump calibration procedure is performed. The pump calibration procedure involves the analysis of a known volume of a known solution. 1 ml of the pump calibration solution is used for the low while 10 ml is used for the high range. The pump calibration procedure should be performed anytime the titrant, syringe, tubing or ORP electrode is replaced. Periodic calibrations should be performed in order to accurately determine the free and total sulfur dioxide levels in wine.
The HI84500-55 is a 120 mL bottle of pump calibration standard solution. The number of calibrations performed will be determined by the range being calibrated. The low range uses 1 ml of the standard and the high range uses 10 ml.
Replacement reagents required to operate the HI84500
Low Range - 1.0 to 40.0 ppm of SO2
High Range - 30 to 400 ppm of SO2
Pump calibration standard
Alkaline reagent (Total SO2)
Stabilizer powder packets
Package bottles Size 120 mL Quantity 1